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Tn5 tagments and transposes oligos to single-stranded DNA for strand-specific RNA sequencing

Tn5 transposon tagments double-stranded DNA and RNA/DNA hybrids to generate nucleic acids that are ready to be amplified for high-throughput sequencing. The nucleic acid substrates for the Tn5 transposon must be explored to increase the applications of Tn5. Here, we found that the Tn5 transposon can...

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Detalles Bibliográficos
Autores principales: Zhang, Yanjun, Tang, Yin, Sun, Zhongxing, Jia, Junqi, Fang, Yuan, Wan, Xinyi, Fang, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10078286/
https://www.ncbi.nlm.nih.gov/pubmed/36958795
http://dx.doi.org/10.1101/gr.277213.122