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Lysosome transporter purification and reconstitution identifies Ypq1 pH-gated lysine transport and regulation
Lysosomes achieve their function through numerous transporters that import or export nutrients across their membrane. However, technical challenges in membrane protein overexpression, purification, and reconstitution hinder our understanding of lysosome transporter function. Here, we developed a pla...
Autores principales: | Arines, Felichi Mae, Wielenga, Aleksander, Burata, Olive E., Garcia, Francisco Narro, Stockbridge, Randy B., Li, Ming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10081341/ https://www.ncbi.nlm.nih.gov/pubmed/37034749 http://dx.doi.org/10.1101/2023.03.31.535002 |
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