Extension of bacterial rDNA sequencing for simultaneous methylation detection and its application in microflora analysis

Although polymerase chain reaction (PCR) amplification and sequencing of the bacterial 16S rDNA region has numerous scientific applications, it does not provide DNA methylation information. Herein, we propose a simple extension for bisulfite sequencing to investigate 5-methylcytosine residues in the...

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Detalles Bibliográficos
Autores principales: Nishimura, Motoi, Tanaka, Tomoaki, Murata, Syota, Miyabe, Akiko, Ishige, Takayuki, Kawasaki, Kenji, Yokoyama, Masataka, Hashimoto, Naoko, Yamagata, Kazuyuki, Nagano, Hidekazu, Tojo-Nishimura, Satomi, Matsushita, Kazuyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10082018/
https://www.ncbi.nlm.nih.gov/pubmed/37029177
http://dx.doi.org/10.1038/s41598-023-28706-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10082018/
https://www.ncbi.nlm.nih.gov/pubmed/37029177
http://dx.doi.org/10.1038/s41598-023-28706-w

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