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Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry

Extracellular vesicles (EVs) are an upcoming biomarker for disease. However, the measured concentrations of EVs by flow cytometry are incomparable due to analytical variables. This study aimed to investigate how the choice of fluorophore, and thereby brightness, affects the measured concentration of...

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Detalles Bibliográficos
Autores principales: Gankema, Angela Adriana Francisca, Li, Bo, Nieuwland, Rienk, van der Pol, Edwin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084316/
https://www.ncbi.nlm.nih.gov/pubmed/35707999
http://dx.doi.org/10.1002/cyto.a.24665