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Automated fluorescence gating and size determination reduce variation in measured concentration of extracellular vesicles by flow cytometry
Extracellular vesicles (EVs) are an upcoming biomarker for disease. However, the measured concentrations of EVs by flow cytometry are incomparable due to analytical variables. This study aimed to investigate how the choice of fluorophore, and thereby brightness, affects the measured concentration of...
Autores principales: | Gankema, Angela Adriana Francisca, Li, Bo, Nieuwland, Rienk, van der Pol, Edwin |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10084316/ https://www.ncbi.nlm.nih.gov/pubmed/35707999 http://dx.doi.org/10.1002/cyto.a.24665 |
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