High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs

T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science to mRNA therapeutics. This in vitro system, while showing high fidelity in many ways, is also well known for producing longer than encoded RNA products, particularly under...

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Detalles Bibliográficos
Autores principales: MalagodaPathiranage, Kithmie, Cavac, Elvan, Chen, Tien-Hao, Roy, Bijoyita, Martin, Craig T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085681/
https://www.ncbi.nlm.nih.gov/pubmed/36718937
http://dx.doi.org/10.1093/nar/gkad027

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085681/
https://www.ncbi.nlm.nih.gov/pubmed/36718937
http://dx.doi.org/10.1093/nar/gkad027

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