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High-salt transcription from enzymatically gapped promoters nets higher yields and purity of transcribed RNAs
T7 RNA polymerase is commonly used to synthesize large quantities of RNA for a wide variety of applications, from basic science to mRNA therapeutics. This in vitro system, while showing high fidelity in many ways, is also well known for producing longer than encoded RNA products, particularly under...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085681/ https://www.ncbi.nlm.nih.gov/pubmed/36718937 http://dx.doi.org/10.1093/nar/gkad027 |