Expanded MutaT7 toolkit efficiently and simultaneously accesses all possible transition mutations in bacteria

Targeted mutagenesis mediated by nucleotide base deaminase–T7 RNA polymerase fusions has recently emerged as a novel and broadly useful strategy to power genetic diversification in the context of in vivo directed evolution campaigns. Here, we expand the utility of this approach by introducing a high...

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Detalles Bibliográficos
Autores principales: Mengiste, Amanuella A, Wilson, Robert H, Weissman, Rachel F, Papa III, Louis J, Hendel, Samuel J, Moore, Christopher L, Butty, Vincent L, Shoulders, Matthew D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085711/
https://www.ncbi.nlm.nih.gov/pubmed/36715334
http://dx.doi.org/10.1093/nar/gkad003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085711/
https://www.ncbi.nlm.nih.gov/pubmed/36715334
http://dx.doi.org/10.1093/nar/gkad003

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