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Expanded MutaT7 toolkit efficiently and simultaneously accesses all possible transition mutations in bacteria
Targeted mutagenesis mediated by nucleotide base deaminase–T7 RNA polymerase fusions has recently emerged as a novel and broadly useful strategy to power genetic diversification in the context of in vivo directed evolution campaigns. Here, we expand the utility of this approach by introducing a high...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10085711/ https://www.ncbi.nlm.nih.gov/pubmed/36715334 http://dx.doi.org/10.1093/nar/gkad003 |