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Sortase A transpeptidation produces seamless, unbranched biotinylated nanobodies for multivalent and multifunctional applications
Exploitation of the biotin–streptavidin interaction for advanced protein engineering is used in many bio-nanotechnology applications. As such, researchers have used diverse techniques involving chemical and enzyme reactions to conjugate biotin to biomolecules of interest for subsequent docking onto...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10089078/ https://www.ncbi.nlm.nih.gov/pubmed/37056610 http://dx.doi.org/10.1039/d3na00014a |