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Sortase A transpeptidation produces seamless, unbranched biotinylated nanobodies for multivalent and multifunctional applications

Exploitation of the biotin–streptavidin interaction for advanced protein engineering is used in many bio-nanotechnology applications. As such, researchers have used diverse techniques involving chemical and enzyme reactions to conjugate biotin to biomolecules of interest for subsequent docking onto...

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Detalles Bibliográficos
Autores principales: Obeng, Eugene M., Steer, David L., Fulcher, Alex J., Wagstaff, Kylie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10089078/
https://www.ncbi.nlm.nih.gov/pubmed/37056610
http://dx.doi.org/10.1039/d3na00014a