Enabling low-cost and robust essentiality studies with high-throughput transposon mutagenesis (HTTM)

Transposon-insertion sequencing (TIS) methods couple high density transposon mutagenesis with next-generation sequencing and are commonly used to identify essential or important genes in bacteria. However, this approach can be work-intensive and sometimes expensive depending on the selected protocol...

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Detalles Bibliográficos
Autores principales: Champie, Antoine, De Grandmaison, Amélie, Jeanneau, Simon, Grenier, Frédéric, Jacques, Pierre-Étienne, Rodrigue, Sébastien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Lab Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10089323/
https://www.ncbi.nlm.nih.gov/pubmed/37040373
http://dx.doi.org/10.1371/journal.pone.0283990

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10089323/
https://www.ncbi.nlm.nih.gov/pubmed/37040373
http://dx.doi.org/10.1371/journal.pone.0283990

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