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High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy

The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (...

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Autores principales: Bianco, Angelica, Normanno, Giovanni, Capozzi, Loredana, Del Sambro, Laura, Di Fato, Laura, Miccolupo, Angela, Di Taranto, Pietro, Caruso, Marta, Petruzzi, Fiorenza, Ali, Ashraf, Parisi, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094235/
https://www.ncbi.nlm.nih.gov/pubmed/37048369
http://dx.doi.org/10.3390/foods12071548
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author Bianco, Angelica
Normanno, Giovanni
Capozzi, Loredana
Del Sambro, Laura
Di Fato, Laura
Miccolupo, Angela
Di Taranto, Pietro
Caruso, Marta
Petruzzi, Fiorenza
Ali, Ashraf
Parisi, Antonio
author_facet Bianco, Angelica
Normanno, Giovanni
Capozzi, Loredana
Del Sambro, Laura
Di Fato, Laura
Miccolupo, Angela
Di Taranto, Pietro
Caruso, Marta
Petruzzi, Fiorenza
Ali, Ashraf
Parisi, Antonio
author_sort Bianco, Angelica
collection PubMed
description The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (s.l.). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the nheABC and the hblCDA gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes entS and entFM; 8% of the isolates possessed the ces gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of B. cereus s.l. in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by B. cereus s.l.
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spelling pubmed-100942352023-04-13 High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy Bianco, Angelica Normanno, Giovanni Capozzi, Loredana Del Sambro, Laura Di Fato, Laura Miccolupo, Angela Di Taranto, Pietro Caruso, Marta Petruzzi, Fiorenza Ali, Ashraf Parisi, Antonio Foods Article The Bacillus cereus group includes species that act as food-borne pathogens causing diarrheal and emetic symptoms. They are widely distributed and can be found in various foods. In this study, out of 550 samples of milk and cheeses, 139 (25.3%) were found to be contaminated by B. cereus sensu lato (s.l.). One isolate per positive sample was characterized by Multilocus Sequence Typing (MLST) and for the presence of ten virulence genes. Based on MLST, all isolates were classified into 73 different sequence types (STs), of which 12 isolates were assigned to new STs. Virulence genes detection revealed that 90% and 61% of the isolates harboured the nheABC and the hblCDA gene cluster, respectively. Ninety-four percent of the isolates harboured the enterotoxin genes entS and entFM; 8% of the isolates possessed the ces gene. Thirty-eight different genetic profiles were identified, suggesting a high genetic diversity. Our study clearly shows the widespread diffusion of potentially toxigenic isolates of B. cereus s.l. in milk and cheeses in the Apulia region highlighting the need to adopt GMP and HACCP procedures along every step of the milk and cheese production chain in order to reduce the public health risk linked to the consumption of foods contaminated by B. cereus s.l. MDPI 2023-04-06 /pmc/articles/PMC10094235/ /pubmed/37048369 http://dx.doi.org/10.3390/foods12071548 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bianco, Angelica
Normanno, Giovanni
Capozzi, Loredana
Del Sambro, Laura
Di Fato, Laura
Miccolupo, Angela
Di Taranto, Pietro
Caruso, Marta
Petruzzi, Fiorenza
Ali, Ashraf
Parisi, Antonio
High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title_full High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title_fullStr High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title_full_unstemmed High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title_short High Genetic Diversity and Virulence Potential in Bacillus cereus sensu lato Isolated from Milk and Cheeses in Apulia Region, Southern Italy
title_sort high genetic diversity and virulence potential in bacillus cereus sensu lato isolated from milk and cheeses in apulia region, southern italy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094235/
https://www.ncbi.nlm.nih.gov/pubmed/37048369
http://dx.doi.org/10.3390/foods12071548
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