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Double FIT hybridization probes – towards enhancing brightness, turn-on and specificity of RNA detection
Efficient fluorogenic hybridization probes combine high brightness and specificity of fluorescence signaling with large turn-on of fluorescence. Herein, we present an approach to enhance signaling by combining two identical fluorescence base surrogates in FIT(2) probes. Provided there is a suitable...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094420/ https://www.ncbi.nlm.nih.gov/pubmed/37063796 http://dx.doi.org/10.1039/d3sc00363a |