Multiplexed Fluorescence Plate Reader In Situ Protein Expression Assay in Apoptotic HepG2 Cells

Instead of Western blot being considered as a gold standard for intracellular protein expression assays, we developed a novel multiplexed high throughput (180 tests/day) in situ manual protein expression method directly in 96-well plates using 25,000–100,000 cells/well after formaldehyde fixation an...

Descripción completa

Detalles Bibliográficos
Autores principales: Jakabfi-Csepregi, Rita, Kovács, Gábor L., Kaltenecker, Péter, Kőszegi, Tamás
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094837/
https://www.ncbi.nlm.nih.gov/pubmed/37047536
http://dx.doi.org/10.3390/ijms24076564

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094837/
https://www.ncbi.nlm.nih.gov/pubmed/37047536
http://dx.doi.org/10.3390/ijms24076564

Ejemplares similares