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Multiplexed Fluorescence Plate Reader In Situ Protein Expression Assay in Apoptotic HepG2 Cells
Instead of Western blot being considered as a gold standard for intracellular protein expression assays, we developed a novel multiplexed high throughput (180 tests/day) in situ manual protein expression method directly in 96-well plates using 25,000–100,000 cells/well after formaldehyde fixation an...
Autores principales: | Jakabfi-Csepregi, Rita, Kovács, Gábor L., Kaltenecker, Péter, Kőszegi, Tamás |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10094837/ https://www.ncbi.nlm.nih.gov/pubmed/37047536 http://dx.doi.org/10.3390/ijms24076564 |
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