Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435

Meleagrin and oxaline, which belong to the roquefortine alkaloids with a unique dihydroindole spiroamide framework, have significant bioactivities, especially tumor cell inhibitory activity. In order to discover the requefortine alkaloids, Penicillium sp. OUCMDZ-1435 was fished and identified from m...

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Autores principales: He, Xingxing, Jin, Yanzheng, Kong, Fei, Yang, Liyuan, Zhu, Mingzhuang, Wang, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095773/
https://www.ncbi.nlm.nih.gov/pubmed/37049942
http://dx.doi.org/10.3390/molecules28073180
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author He, Xingxing
Jin, Yanzheng
Kong, Fei
Yang, Liyuan
Zhu, Mingzhuang
Wang, Yi
author_facet He, Xingxing
Jin, Yanzheng
Kong, Fei
Yang, Liyuan
Zhu, Mingzhuang
Wang, Yi
author_sort He, Xingxing
collection PubMed
description Meleagrin and oxaline, which belong to the roquefortine alkaloids with a unique dihydroindole spiroamide framework, have significant bioactivities, especially tumor cell inhibitory activity. In order to discover the requefortine alkaloids, Penicillium sp. OUCMDZ-1435 was fished and identified from marine fungi using molecular probe technology. Meleagrin (1) and oxaline (2) were isolated from it. In addition, we first reported that compounds 1 and 2 could effectively inhibit the proliferation and metastasis of the human HepG2 cell and induce HepG2 cell apoptosis and cell cycle arrest in the G2/M phase. Additionally, the fermentation of Meleagrin (1) was optimized to increase its yield to 335 mg/L. These results provided bioactive inspiration and fungus resources for roquefortine alkaloid development.
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spelling pubmed-100957732023-04-13 Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435 He, Xingxing Jin, Yanzheng Kong, Fei Yang, Liyuan Zhu, Mingzhuang Wang, Yi Molecules Article Meleagrin and oxaline, which belong to the roquefortine alkaloids with a unique dihydroindole spiroamide framework, have significant bioactivities, especially tumor cell inhibitory activity. In order to discover the requefortine alkaloids, Penicillium sp. OUCMDZ-1435 was fished and identified from marine fungi using molecular probe technology. Meleagrin (1) and oxaline (2) were isolated from it. In addition, we first reported that compounds 1 and 2 could effectively inhibit the proliferation and metastasis of the human HepG2 cell and induce HepG2 cell apoptosis and cell cycle arrest in the G2/M phase. Additionally, the fermentation of Meleagrin (1) was optimized to increase its yield to 335 mg/L. These results provided bioactive inspiration and fungus resources for roquefortine alkaloid development. MDPI 2023-04-03 /pmc/articles/PMC10095773/ /pubmed/37049942 http://dx.doi.org/10.3390/molecules28073180 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
He, Xingxing
Jin, Yanzheng
Kong, Fei
Yang, Liyuan
Zhu, Mingzhuang
Wang, Yi
Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title_full Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title_fullStr Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title_full_unstemmed Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title_short Discovery, Antitumor Activity, and Fermentation Optimization of Roquefortines from Penicillium sp. OUCMDZ-1435
title_sort discovery, antitumor activity, and fermentation optimization of roquefortines from penicillium sp. oucmdz-1435
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10095773/
https://www.ncbi.nlm.nih.gov/pubmed/37049942
http://dx.doi.org/10.3390/molecules28073180
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