Alteration of Substrate Specificity and Transglucosylation Activity of GH13_31 α-Glucosidase from Bacillus sp. AHU2216 through Site-Directed Mutagenesis of Asn258 on β→α Loop 5

α-Glucosidase catalyzes the hydrolysis of α-d-glucosides and transglucosylation. Bacillus sp. AHU2216 α-glucosidase (BspAG13_31A), belonging to the glycoside hydrolase family 13 subfamily 31, specifically cleaves α-(1→4)-glucosidic linkages and shows high disaccharide specificity. We showed previous...

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Detalles Bibliográficos
Autores principales: Auiewiriyanukul, Waraporn, Saburi, Wataru, Ota, Tomoya, Yu, Jian, Kato, Koji, Yao, Min, Mori, Haruhide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096246/
https://www.ncbi.nlm.nih.gov/pubmed/37049872
http://dx.doi.org/10.3390/molecules28073109

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10096246/
https://www.ncbi.nlm.nih.gov/pubmed/37049872
http://dx.doi.org/10.3390/molecules28073109

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