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RNA Probes for Visualization of Sarcin/ricin Loop Depurination without Background Fluorescence

Protein synthesis via ribosomes is a fundamental process in all known living organisms. However, it can be completely stalled by removing a single nucleobase (depurination) at the sarcin/ricin loop of the ribosomal RNA. In this work, we describe the preparation and optimization process of a fluoresc...

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Detalles Bibliográficos
Autores principales: Klimek, Robin, Kaiser, Christoph, Murmann, Nina S., Kaltenschnee, Nina, Spanò, Teresa, Wachtveitl, Josef, Schuman, Erin M., Heckel, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10098603/
https://www.ncbi.nlm.nih.gov/pubmed/36321802
http://dx.doi.org/10.1002/asia.202201077