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Circularization of rv0678 for Genotypic Bedaquiline Resistance Testing of Mycobacterium tuberculosis

Circular DNA offers benefits over linear DNA in diagnostic and field assays, but currently, circular DNA generation is lengthy, inefficient, highly dependent on the length and sequence of DNA, and can result in unwanted chimeras. We present streamlined methods for generating PCR-targeted circular DN...

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Detalles Bibliográficos
Autores principales: Limberis, Jason D., Nalyvayko, Alina, Ernst, Joel D., Metcalfe, John Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10100719/
https://www.ncbi.nlm.nih.gov/pubmed/36877083
http://dx.doi.org/10.1128/spectrum.04127-22