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Impact of 16S rRNA Gene Redundancy and Primer Pair Selection on the Quantification and Classification of Oral Microbiota in Next-Generation Sequencing

This study aimed to evaluate the number of 16S rRNA genes in the complete genomes of the bacterial and archaeal species inhabiting the human mouth and to assess how the use of different primer pairs would affect the detection and classification of redundant amplicons and matching amplicons (MAs) fro...

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Detalles Bibliográficos
Autores principales: Regueira-Iglesias, Alba, Vázquez-González, Lara, Balsa-Castro, Carlos, Blanco-Pintos, Triana, Vila-Blanco, Nicolás, Carreira, Maria José, Tomás, Inmaculada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101033/
https://www.ncbi.nlm.nih.gov/pubmed/36779795
http://dx.doi.org/10.1128/spectrum.04398-22