Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis

Phytase efficiently hydrolyzes phytate to phosphate; thus, it is widely used to increase phosphorus availability in animal feeds and reduce phosphorus pollution through excretion. Phytase is easily inactivated during feed pelleting at high temperature, and sufficient thermostability of phytase is es...

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Detalles Bibliográficos
Autores principales: Xing, Hongguan, Wang, Pingping, Yan, Xing, Yang, Yi, Li, Xinliang, Liu, Rui, Zhou, Zhihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101328/
https://www.ncbi.nlm.nih.gov/pubmed/37064242
http://dx.doi.org/10.3389/fbioe.2023.1167530

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10101328/
https://www.ncbi.nlm.nih.gov/pubmed/37064242
http://dx.doi.org/10.3389/fbioe.2023.1167530

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