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pOpsicle: An all-optical reporter system for synaptic vesicle recycling combining pH-sensitive fluorescent proteins with optogenetic manipulation of neuronal activity
pH-sensitive fluorescent proteins are widely used to study synaptic vesicle (SV) fusion and recycling. When targeted to the lumen of SVs, fluorescence of these proteins is quenched by the acidic pH. Following SV fusion, they are exposed to extracellular neutral pH, resulting in a fluorescence increa...
Autores principales: | Seidenthal, Marius, Jánosi, Barbara, Rosenkranz, Nils, Schuh, Noah, Elvers, Nora, Willoughby, Miles, Zhao, Xinda, Gottschalk, Alexander |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10102542/ https://www.ncbi.nlm.nih.gov/pubmed/37066081 http://dx.doi.org/10.3389/fncel.2023.1120651 |
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