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A DNA assembly toolkit to unlock the CRISPR/Cas9 potential for metabolic engineering
CRISPR/Cas9-based technologies are revolutionising the way we engineer microbial cells. One of the key advantages of CRISPR in strain design is that it enables chromosomal integration of marker-free DNA, eliminating laborious and often inefficient marker recovery procedures. Despite the benefits, as...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Journal Experts
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10104256/ https://www.ncbi.nlm.nih.gov/pubmed/37066237 http://dx.doi.org/10.21203/rs.3.rs-2738543/v1 |