Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens

The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme e...

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Detalles Bibliográficos
Autores principales: Gu, Binbin, Goldfuss, Bernd, Dickschat, Jeroen S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107272/
https://www.ncbi.nlm.nih.gov/pubmed/36350768
http://dx.doi.org/10.1002/anie.202215688
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author Gu, Binbin
Goldfuss, Bernd
Dickschat, Jeroen S.
author_facet Gu, Binbin
Goldfuss, Bernd
Dickschat, Jeroen S.
author_sort Gu, Binbin
collection PubMed
description The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme engineering through site‐directed mutagenesis gave access to a high‐yielding enzyme variant that provided six additional minor products and the main product in sufficient quantities to study its chemistry.
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spelling pubmed-101072722023-04-18 Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens Gu, Binbin Goldfuss, Bernd Dickschat, Jeroen S. Angew Chem Int Ed Engl Communications The sesterviolene synthase from Streptomyces violens was identified and represents the second known sesterterpene synthase from bacteria. Isotopic labelling experiments in conjunction with DFT calculations were performed that provided detailed insight into its complex cyclisation mechanism. Enzyme engineering through site‐directed mutagenesis gave access to a high‐yielding enzyme variant that provided six additional minor products and the main product in sufficient quantities to study its chemistry. John Wiley and Sons Inc. 2022-12-01 2023-01-02 /pmc/articles/PMC10107272/ /pubmed/36350768 http://dx.doi.org/10.1002/anie.202215688 Text en © 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Communications
Gu, Binbin
Goldfuss, Bernd
Dickschat, Jeroen S.
Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title_full Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title_fullStr Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title_full_unstemmed Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title_short Mechanistic Characterisation and Engineering of Sesterviolene Synthase from Streptomyces violens
title_sort mechanistic characterisation and engineering of sesterviolene synthase from streptomyces violens
topic Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107272/
https://www.ncbi.nlm.nih.gov/pubmed/36350768
http://dx.doi.org/10.1002/anie.202215688
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