Enzymatic C4‐Epimerization of UDP‐Glucuronic Acid: Precisely Steered Rotation of a Transient 4‐Keto Intermediate for an Inverted Reaction without Decarboxylation

UDP‐glucuronic acid (UDP‐GlcA) 4‐epimerase illustrates an important problem regarding enzyme catalysis: balancing conformational flexibility with precise positioning. The enzyme coordinates the C4‐oxidation of the substrate by NAD(+) and rotation of a decarboxylation‐prone β‐keto acid intermediate i...

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Detalles Bibliográficos
Autores principales: Borg, Annika J. E., Esquivias, Oriol, Coines, Joan, Rovira, Carme, Nidetzky, Bernd
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107529/
https://www.ncbi.nlm.nih.gov/pubmed/36308301
http://dx.doi.org/10.1002/anie.202211937

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10107529/
https://www.ncbi.nlm.nih.gov/pubmed/36308301
http://dx.doi.org/10.1002/anie.202211937

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