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Using RNA-seq to identify suitable housekeeping genes for hypoxia studies in human adipose-derived stem cells

BACKGROUND: Hypoxic culture conditions have been used to study the impact of oxygen deprivation has on gene expression in a number of disease models. However, hypoxia response elements present in the promoter regions of some commonly used housekeeping genes, such as GAPDH and PGK1, can confound the...

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Detalles Bibliográficos
Autores principales: Ong, Huan Ting, Prêle, Cecilia M., Dilley, Rodney J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10108514/
https://www.ncbi.nlm.nih.gov/pubmed/37062833
http://dx.doi.org/10.1186/s12860-023-00475-4