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Easy efficient HDR-based targeted knock-in in Saccharomyces cerevisiae genome using CRISPR-Cas9 system

During the last two decades, yeast has been used as a biological tool to produce various small molecules, biofuels, etc., using an inexpensive bioprocess. The application of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated protein (Cas) techniques in yeast genetic...

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Detalles Bibliográficos
Autores principales: Singh, Rajveer, Chandel, Shivani, Ghosh, Arijit, Gautam, Anupam, Huson, Daniel H., Ravichandiran, V., Ghosh, Dipanjan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10109214/
https://www.ncbi.nlm.nih.gov/pubmed/36602175
http://dx.doi.org/10.1080/21655979.2022.2162667