Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses
Metagenomic studies have demonstrated that viruses are extremely diverse and abundant in insects, but the difficulty of isolating them means little is known about the biology of these newly discovered viruses. To overcome this challenge in Drosophila, we created a cell line that was more permissive...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10109509/ https://www.ncbi.nlm.nih.gov/pubmed/36996192 http://dx.doi.org/10.1371/journal.ppat.1010883 |
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author | Bruner-Montero, Gaspar Luque, Carlos M. Cesar, Cássia Siqueira Ding, Shuai Dominique Day, Jonathan P. Jiggins, Francis Michael |
author_facet | Bruner-Montero, Gaspar Luque, Carlos M. Cesar, Cássia Siqueira Ding, Shuai Dominique Day, Jonathan P. Jiggins, Francis Michael |
author_sort | Bruner-Montero, Gaspar |
collection | PubMed |
description | Metagenomic studies have demonstrated that viruses are extremely diverse and abundant in insects, but the difficulty of isolating them means little is known about the biology of these newly discovered viruses. To overcome this challenge in Drosophila, we created a cell line that was more permissive to infection and detected novel viruses by the presence of double-stranded RNA. We demonstrate the utility of these tools by isolating La Jolla virus (LJV) and Newfield virus (NFV) from several wild Drosophila populations. These viruses have different potential host ranges, with distinct abilities to replicate in five Drosophila species. Similarly, in some species they cause high mortality and in others they are comparatively benign. In three species, NFV but not LJV caused large declines in female fecundity. This sterilization effect was associated with differences in tissue tropism, as NFV but not LJV was able to infect Drosophila melanogaster follicular epithelium and induce follicular degeneration in the ovary. We saw a similar effect in the invasive pest of fruit crops Drosophila suzukii, where oral infection with NFV caused reductions in the fecundity, suggesting it has potential as a biocontrol agent. In conclusion, a simple protocol allowed us to isolate new viruses and demonstrate that viruses identified by metagenomics have a large effect on the fitness of the model organism D. melanogaster and related species. |
format | Online Article Text |
id | pubmed-10109509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-101095092023-04-18 Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses Bruner-Montero, Gaspar Luque, Carlos M. Cesar, Cássia Siqueira Ding, Shuai Dominique Day, Jonathan P. Jiggins, Francis Michael PLoS Pathog Research Article Metagenomic studies have demonstrated that viruses are extremely diverse and abundant in insects, but the difficulty of isolating them means little is known about the biology of these newly discovered viruses. To overcome this challenge in Drosophila, we created a cell line that was more permissive to infection and detected novel viruses by the presence of double-stranded RNA. We demonstrate the utility of these tools by isolating La Jolla virus (LJV) and Newfield virus (NFV) from several wild Drosophila populations. These viruses have different potential host ranges, with distinct abilities to replicate in five Drosophila species. Similarly, in some species they cause high mortality and in others they are comparatively benign. In three species, NFV but not LJV caused large declines in female fecundity. This sterilization effect was associated with differences in tissue tropism, as NFV but not LJV was able to infect Drosophila melanogaster follicular epithelium and induce follicular degeneration in the ovary. We saw a similar effect in the invasive pest of fruit crops Drosophila suzukii, where oral infection with NFV caused reductions in the fecundity, suggesting it has potential as a biocontrol agent. In conclusion, a simple protocol allowed us to isolate new viruses and demonstrate that viruses identified by metagenomics have a large effect on the fitness of the model organism D. melanogaster and related species. Public Library of Science 2023-03-30 /pmc/articles/PMC10109509/ /pubmed/36996192 http://dx.doi.org/10.1371/journal.ppat.1010883 Text en © 2023 Bruner-Montero et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Bruner-Montero, Gaspar Luque, Carlos M. Cesar, Cássia Siqueira Ding, Shuai Dominique Day, Jonathan P. Jiggins, Francis Michael Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title | Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title_full | Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title_fullStr | Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title_full_unstemmed | Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title_short | Hunting Drosophila viruses from wild populations: A novel isolation approach and characterisation of viruses |
title_sort | hunting drosophila viruses from wild populations: a novel isolation approach and characterisation of viruses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10109509/ https://www.ncbi.nlm.nih.gov/pubmed/36996192 http://dx.doi.org/10.1371/journal.ppat.1010883 |
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