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De novo PAM generation to reach initially inaccessible target sites for base editing

Base editing by CRISPR crucially depends on the presence of a protospacer adjacent motif (PAM) at the correct distance from the editing site. Here, we present and validate an efficient one-shot approach termed ‘inception’ that expands the editing range. This is achieved by sequential, combinatorial...

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Detalles Bibliográficos
Autores principales: Pakari, Kaisa, Wittbrodt, Joachim, Thumberger, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10110497/
https://www.ncbi.nlm.nih.gov/pubmed/36683434
http://dx.doi.org/10.1242/dev.201115