Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors

To date, the establishment of high-titer stable viral packaging cells (VPCs) at large scale for gene therapeutic applications is very time- and cost-intensive. Here we report the establishment of three human suspension 293-F-derived ecotropic MLV-based VPCs. The classic stable transfection of an EGF...

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Detalles Bibliográficos
Autores principales: van Heuvel, Yasemin, Schatz, Stefanie, Hein, Marc, Dogra, Tanya, Kazenmaier, Daniel, Tschorn, Natalie, Genzel, Yvonne, Stitz, Jörn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10112512/
https://www.ncbi.nlm.nih.gov/pubmed/37082212
http://dx.doi.org/10.3389/fbioe.2023.1076524

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10112512/
https://www.ncbi.nlm.nih.gov/pubmed/37082212
http://dx.doi.org/10.3389/fbioe.2023.1076524

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