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βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression
Opioid medications are the mainstay of pain management but present substantial side-effects such as respiratory depression which can be lethal with overdose. Most opioid drugs, such as fentanyl, act on opioid receptors such as the G-protein-coupled µ-opioid receptors (MOR). G-protein-coupled recepto...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117644/ https://www.ncbi.nlm.nih.gov/pubmed/37089428 http://dx.doi.org/10.3389/fphys.2023.1043581 |
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author | Danaf, Jamil da Silveira Scarpellini, Carolina Montandon, Gaspard |
author_facet | Danaf, Jamil da Silveira Scarpellini, Carolina Montandon, Gaspard |
author_sort | Danaf, Jamil |
collection | PubMed |
description | Opioid medications are the mainstay of pain management but present substantial side-effects such as respiratory depression which can be lethal with overdose. Most opioid drugs, such as fentanyl, act on opioid receptors such as the G-protein-coupled µ-opioid receptors (MOR). G-protein-coupled receptors activate pertussis toxin-sensitive G-proteins to inhibit neuronal activity. Binding of opioid ligands to MOR and subsequent activation G proteins βγ is modulated by regulator of G-protein signaling (RGS). The roles of G-proteins βγ and RGS in MOR-mediated inhibition of the respiratory network are not known. Using rodent models to pharmacologically modulate G-protein signaling, we aim to determine the roles of βγ G-proteins and RGS4. We showed that inhibition of βγ G-proteins using gallein perfused in the brainstem circuits regulating respiratory depression by opioid drugs results in complete reversal of respiratory depression. Blocking of RGS4 using CCG55014 did not change the respiratory depression induced by MOR activation despite co-expression of RGS4 and MORs in the brainstem. Our results suggest that neuronal inhibition by opioid drugs is mediated by G-proteins, but not by RGS4, which supports the concept that βγ G-proteins could be molecular targets to develop opioid overdose antidotes without the risks of re-narcotization often found with highly potent opioid drugs. On the other hand, RGS4 mediates opioid analgesia, but not respiratory depression, and RGS4 may be molecular targets to develop pain therapies without respiratory liability. |
format | Online Article Text |
id | pubmed-10117644 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-101176442023-04-21 βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression Danaf, Jamil da Silveira Scarpellini, Carolina Montandon, Gaspard Front Physiol Physiology Opioid medications are the mainstay of pain management but present substantial side-effects such as respiratory depression which can be lethal with overdose. Most opioid drugs, such as fentanyl, act on opioid receptors such as the G-protein-coupled µ-opioid receptors (MOR). G-protein-coupled receptors activate pertussis toxin-sensitive G-proteins to inhibit neuronal activity. Binding of opioid ligands to MOR and subsequent activation G proteins βγ is modulated by regulator of G-protein signaling (RGS). The roles of G-proteins βγ and RGS in MOR-mediated inhibition of the respiratory network are not known. Using rodent models to pharmacologically modulate G-protein signaling, we aim to determine the roles of βγ G-proteins and RGS4. We showed that inhibition of βγ G-proteins using gallein perfused in the brainstem circuits regulating respiratory depression by opioid drugs results in complete reversal of respiratory depression. Blocking of RGS4 using CCG55014 did not change the respiratory depression induced by MOR activation despite co-expression of RGS4 and MORs in the brainstem. Our results suggest that neuronal inhibition by opioid drugs is mediated by G-proteins, but not by RGS4, which supports the concept that βγ G-proteins could be molecular targets to develop opioid overdose antidotes without the risks of re-narcotization often found with highly potent opioid drugs. On the other hand, RGS4 mediates opioid analgesia, but not respiratory depression, and RGS4 may be molecular targets to develop pain therapies without respiratory liability. Frontiers Media S.A. 2023-04-06 /pmc/articles/PMC10117644/ /pubmed/37089428 http://dx.doi.org/10.3389/fphys.2023.1043581 Text en Copyright © 2023 Danaf, da Silveira Scarpellini and Montandon. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Danaf, Jamil da Silveira Scarpellini, Carolina Montandon, Gaspard βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title | βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title_full | βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title_fullStr | βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title_full_unstemmed | βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title_short | βγ G-proteins, but not regulators of G-protein signaling 4, modulate opioid-induced respiratory rate depression |
title_sort | βγ g-proteins, but not regulators of g-protein signaling 4, modulate opioid-induced respiratory rate depression |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117644/ https://www.ncbi.nlm.nih.gov/pubmed/37089428 http://dx.doi.org/10.3389/fphys.2023.1043581 |
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