Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas

Pyruvate carboxylase (PC) is an enzyme catalyzing the carboxylation of pyruvate to oxaloacetate. The enzymatic generation of oxaloacetate, an intermediate of the Krebs cycle, could provide the cancer cells with the additional anaplerotic capacity and promote their anabolic metabolism. Recent studies...

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Autores principales: Gondáš, Eduard, Kráľová Trančíková, Alžbeta, Dibdiaková, Katarina, Galanda, Tomáš, Hatok, Jozef, Račay, Peter, Dobrota, Dušan, Murín, Radovan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2023
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Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119210/
https://www.ncbi.nlm.nih.gov/pubmed/36662405
http://dx.doi.org/10.1007/s11064-023-03856-5
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author Gondáš, Eduard
Kráľová Trančíková, Alžbeta
Dibdiaková, Katarina
Galanda, Tomáš
Hatok, Jozef
Račay, Peter
Dobrota, Dušan
Murín, Radovan
author_facet Gondáš, Eduard
Kráľová Trančíková, Alžbeta
Dibdiaková, Katarina
Galanda, Tomáš
Hatok, Jozef
Račay, Peter
Dobrota, Dušan
Murín, Radovan
author_sort Gondáš, Eduard
collection PubMed
description Pyruvate carboxylase (PC) is an enzyme catalyzing the carboxylation of pyruvate to oxaloacetate. The enzymatic generation of oxaloacetate, an intermediate of the Krebs cycle, could provide the cancer cells with the additional anaplerotic capacity and promote their anabolic metabolism. Recent studies revealed that several types of cancer cells express PC. The gained anaplerotic capability of cells mediated by PC correlates with their expedited growth, higher aggressiveness, and increased metastatic potential. By immunohistochemical staining and immunoblotting analysis, we investigated PC expression among samples of different types of human brain tumors. Our results show that PC is expressed by the cells in glioblastoma, astrocytoma, oligodendroglioma, and meningioma tumors. The presence of PC in these tumors suppose that PC could support the anabolic metabolism of their cellular constituents by its anaplerotic capability. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11064-023-03856-5.
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spelling pubmed-101192102023-04-22 Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas Gondáš, Eduard Kráľová Trančíková, Alžbeta Dibdiaková, Katarina Galanda, Tomáš Hatok, Jozef Račay, Peter Dobrota, Dušan Murín, Radovan Neurochem Res Original Paper Pyruvate carboxylase (PC) is an enzyme catalyzing the carboxylation of pyruvate to oxaloacetate. The enzymatic generation of oxaloacetate, an intermediate of the Krebs cycle, could provide the cancer cells with the additional anaplerotic capacity and promote their anabolic metabolism. Recent studies revealed that several types of cancer cells express PC. The gained anaplerotic capability of cells mediated by PC correlates with their expedited growth, higher aggressiveness, and increased metastatic potential. By immunohistochemical staining and immunoblotting analysis, we investigated PC expression among samples of different types of human brain tumors. Our results show that PC is expressed by the cells in glioblastoma, astrocytoma, oligodendroglioma, and meningioma tumors. The presence of PC in these tumors suppose that PC could support the anabolic metabolism of their cellular constituents by its anaplerotic capability. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11064-023-03856-5. Springer US 2023-01-20 2023 /pmc/articles/PMC10119210/ /pubmed/36662405 http://dx.doi.org/10.1007/s11064-023-03856-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Gondáš, Eduard
Kráľová Trančíková, Alžbeta
Dibdiaková, Katarina
Galanda, Tomáš
Hatok, Jozef
Račay, Peter
Dobrota, Dušan
Murín, Radovan
Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title_full Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title_fullStr Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title_full_unstemmed Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title_short Immunodetection of Pyruvate Carboxylase Expression in Human Astrocytomas, Glioblastomas, Oligodendrogliomas, and Meningiomas
title_sort immunodetection of pyruvate carboxylase expression in human astrocytomas, glioblastomas, oligodendrogliomas, and meningiomas
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119210/
https://www.ncbi.nlm.nih.gov/pubmed/36662405
http://dx.doi.org/10.1007/s11064-023-03856-5
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