Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target

The design of minimum CRISPR RNA (crRNA) sets for detection of diverse RNA targets using sequence degeneracy has not been systematically addressed. We tested candidate degenerate Cas13a crRNA sets designed for detection of diverse RNA targets (Lassa virus). A decision tree machine learning (ML) algo...

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Detalles Bibliográficos
Autores principales: Leski, T. A., Spangler, J. R., Wang, Z., Schultzhaus, Z., Taitt, C. R., Dean, S. N., Stenger, D. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119381/
https://www.ncbi.nlm.nih.gov/pubmed/37081092
http://dx.doi.org/10.1038/s41598-023-33494-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119381/
https://www.ncbi.nlm.nih.gov/pubmed/37081092
http://dx.doi.org/10.1038/s41598-023-33494-4

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