Cargando…
Machine learning for design of degenerate Cas13a crRNAs using lassa virus as a model of highly variable RNA target
The design of minimum CRISPR RNA (crRNA) sets for detection of diverse RNA targets using sequence degeneracy has not been systematically addressed. We tested candidate degenerate Cas13a crRNA sets designed for detection of diverse RNA targets (Lassa virus). A decision tree machine learning (ML) algo...
Autores principales: | Leski, T. A., Spangler, J. R., Wang, Z., Schultzhaus, Z., Taitt, C. R., Dean, S. N., Stenger, D. A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10119381/ https://www.ncbi.nlm.nih.gov/pubmed/37081092 http://dx.doi.org/10.1038/s41598-023-33494-4 |
Ejemplares similares
-
Structural constraints and enzymatic promiscuity in the Cas6-dependent generation of crRNAs
por: Reimann, Viktoria, et al.
Publicado: (2017) -
Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system
por: Spangler, J. R., et al.
Publicado: (2022) -
CRISPR-Cas-Docker: web-based in silico docking and machine learning-based classification of crRNAs with Cas proteins
por: Park, Ho-min, et al.
Publicado: (2023) -
PrimedSherlock: a tool for rapid design of highly specific CRISPR-Cas12 crRNAs
por: Mann, James G., et al.
Publicado: (2022) -
Noncanonical crRNAs derived from host transcripts enable multiplexable RNA detection by Cas9
por: Jiao, Chunlei, et al.
Publicado: (2021)