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Barcode-free multiplex plasmid sequencing using Bayesian analysis and nanopore sequencing

Plasmid construction is central to life science research, and sequence verification is arguably its costliest step. Long-read sequencing has emerged as a competitor to Sanger sequencing, with the principal benefit that whole plasmids can be sequenced in a single run. Nevertheless, the current cost o...

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Detalles Bibliográficos
Autores principales: Uematsu, Masaaki, Baskin, Jeremy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10120676/
https://www.ncbi.nlm.nih.gov/pubmed/37090656
http://dx.doi.org/10.1101/2023.04.12.536413