SMAP design: a multiplex PCR amplicon and gRNA design tool to screen for natural and CRISPR-induced genetic variation

Multiplex amplicon sequencing is a versatile method to identify genetic variation in natural or mutagenized populations through eco-tilling or multiplex CRISPR screens. Such genotyping screens require reliable and specific primer designs, combined with simultaneous gRNA design for CRISPR screens. Un...

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Detalles Bibliográficos
Autores principales: Develtere, Ward, Waegneer, Evelien, Debray, Kevin, De Saeger, Jonas, Van Glabeke, Sabine, Maere, Steven, Ruttink, Tom, Jacobs, Thomas B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123101/
https://www.ncbi.nlm.nih.gov/pubmed/36718951
http://dx.doi.org/10.1093/nar/gkad036

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123101/
https://www.ncbi.nlm.nih.gov/pubmed/36718951
http://dx.doi.org/10.1093/nar/gkad036

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