Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants

Homology-directed recombination (HDR) between donor constructs and acceptor genomic sequences cleaved by programmable nucleases, permits installing large genomic edits in mammalian cells in a precise fashion. Yet, next to precise gene knock-ins, programmable nucleases yield unintended genomic modifi...

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Detalles Bibliográficos
Autores principales: Wang, Qian, Liu, Jin, Janssen, Josephine M, Gonçalves, Manuel A F V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123109/
https://www.ncbi.nlm.nih.gov/pubmed/36928106
http://dx.doi.org/10.1093/nar/gkad165

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123109/
https://www.ncbi.nlm.nih.gov/pubmed/36928106
http://dx.doi.org/10.1093/nar/gkad165

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