Cargando…

Precise homology-directed installation of large genomic edits in human cells with cleaving and nicking high-specificity Cas9 variants

Homology-directed recombination (HDR) between donor constructs and acceptor genomic sequences cleaved by programmable nucleases, permits installing large genomic edits in mammalian cells in a precise fashion. Yet, next to precise gene knock-ins, programmable nucleases yield unintended genomic modifi...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Qian, Liu, Jin, Janssen, Josephine M, Gonçalves, Manuel A F V
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2023
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123109/ https://www.ncbi.nlm.nih.gov/pubmed/36928106 http://dx.doi.org/10.1093/nar/gkad165

Ejemplares similares

Expanding the editable genome and CRISPR–Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking
por: Chen, Xiaoyu, et al.
Publicado: (2020)

Precise and broad scope genome editing based on high-specificity Cas9 nickases
por: Wang, Qian, et al.
Publicado: (2021)

Enabling large-scale genome editing at repetitive elements by reducing DNA nicking
por: Smith, Cory J, et al.
Publicado: (2020)

Large-scale genome editing based on high-capacity adenovectors and CRISPR-Cas9 nucleases rescues full-length dystrophin synthesis in DMD muscle cells
por: Tasca, Francesca, et al.
Publicado: (2022)

High-throughput antibody engineering in mammalian cells by CRISPR/Cas9-mediated homology-directed mutagenesis
por: Mason, Derek M, et al.
Publicado: (2018)

Engineering of temperature- and light-switchable Cas9 variants
por: Richter, Florian, et al.
Publicado: (2016)

Rational guide RNA engineering for small-molecule control of CRISPR/Cas9 and gene editing
por: Liu, Xingyu, et al.
Publicado: (2022)

Stretching DNA origami: effect of nicks and Holliday junctions on the axial stiffness
por: Jung, Wei-Hung, et al.
Publicado: (2020)

Probing the impact of chromatin conformation on genome editing tools
por: Chen, Xiaoyu, et al.
Publicado: (2016)

A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells
por: Carusillo, Antonio, et al.
Publicado: (2023)

Highly efficient ‘hit-and-run’ genome editing with unconcentrated lentivectors carrying Vpr.Prot.Cas9 protein produced from RRE-containing transcripts
por: Indikova, Ivana, et al.
Publicado: (2020)

Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification
por: Haldrup, Jakob, et al.
Publicado: (2023)

CRISPR–Cas12a system in fission yeast for multiplex genomic editing and CRISPR interference
por: Zhao, Yu, et al.
Publicado: (2020)

Metabolically-targeted dCas9 expression in bacteria
por: Pellegrino, Gregory M, et al.
Publicado: (2023)

CRISPR-based self-cleaving mechanism for controllable gene delivery in human cells
por: Moore, Richard, et al.
Publicado: (2015)

CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
por: Yuen, Garmen, et al.
Publicado: (2017)

A Cas9-based toolkit to program gene expression in Saccharomyces cerevisiae
por: Reider Apel, Amanda, et al.
Publicado: (2017)

A tightly regulated and adjustable CRISPR-dCas9 based AND gate in yeast
por: Hofmann, Anja, et al.
Publicado: (2019)

Engineered dCas9 with reduced toxicity in bacteria: implications for genetic circuit design
por: Zhang, Shuyi, et al.
Publicado: (2018)

Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity
por: Rostain, William, et al.
Publicado: (2023)

Precise genome editing of the Kozak sequence enables bidirectional and quantitative modulation of protein translation to anticipated levels without affecting transcription
por: Xie, Jingke, et al.
Publicado: (2023)

A functional type II-A CRISPR–Cas system from Listeria enables efficient genome editing of large non-integrating bacteriophage
por: Hupfeld, Mario, et al.
Publicado: (2018)

Utilizing RNA origami scaffolds in Saccharomyces cerevisiae for dCas9-mediated transcriptional control
por: Pothoulakis, Georgios, et al.
Publicado: (2022)

Establishing artificial gene connections through RNA displacement–assembly-controlled CRISPR/Cas9 function
por: Wang, Wei-Jia, et al.
Publicado: (2023)

Characterization of a promiscuous DNA sulfur binding domain and application in site-directed RNA base editing
por: Hu, Wenyue, et al.
Publicado: (2023)

An in-library ligation strategy and its application in CRISPR/Cas9 screening of high-order gRNA combinations
por: Lu, Zhike, et al.
Publicado: (2022)

Distinct genetic control of homologous recombination repair of Cas9-induced double-strand breaks, nicks and paired nicks
por: Vriend, Lianne E.M., et al.
Publicado: (2016)

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
por: O'Geen, Henriette, et al.
Publicado: (2015)

Enhancing site-specific DNA integration by a Cas9 nuclease fused with a DNA donor-binding domain
por: Ma, Shufeng, et al.
Publicado: (2020)

MiniCAFE, a CRISPR/Cas9-based compact and potent transcriptional activator, elicits gene expression in vivo
por: Zhang, Xin, et al.
Publicado: (2021)

Activating cryptic biosynthetic gene cluster through a CRISPR–Cas12a-mediated direct cloning approach
por: Liang, Mindong, et al.
Publicado: (2022)

Broadening the reach and investigating the potential of prime editors through fully viral gene-deleted adenoviral vector delivery
por: Wang, Qian, et al.
Publicado: (2021)

Selection-free gene repair after adenoviral vector transduction of designer nucleases: rescue of dystrophin synthesis in DMD muscle cell populations
por: Maggio, Ignazio, et al.
Publicado: (2016)

The Chromatin Structure of CRISPR-Cas9 Target DNA Controls the Balance between Mutagenic and Homology-Directed Gene-Editing Events
por: Janssen, Josephine M., et al.
Publicado: (2019)

Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions
por: Lin, Jiao, et al.
Publicado: (2020)

The contribution of homology arms to nuclease-assisted genome engineering
por: Baker, Oliver, et al.
Publicado: (2017)

Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes in Escherichia coli
por: Cress, Brady F., et al.
Publicado: (2016)

Specific targeting of plasmids with Argonaute enables genome editing
por: Esyunina, Daria, et al.
Publicado: (2023)

Unbiased investigation of specificities of prime editing systems in human cells
por: Kim, Do Yon, et al.
Publicado: (2020)

Translational enhancement by base editing of the Kozak sequence rescues haploinsufficiency
por: Ambrosini, Chiara, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_psml7ub1djvq8dsaqi1mmbq9t3