Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes

Regulation of mRNA translation in astrocytes gains a growing interest. However, until now, successful ribosome profiling of primary astrocytes has not been reported. Here, we optimized the standard ‘polysome profiling’ method and generated an effective protocol for polyribosome extraction, which ena...

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Detalles Bibliográficos
Autores principales: Mandelboum, Shir, Herrero, Melisa, Atzmon, Andrea, Ehrlich, Marcelo, Elroy-Stein, Orna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123121/
https://www.ncbi.nlm.nih.gov/pubmed/36881761
http://dx.doi.org/10.1093/nar/gkad131

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123121/
https://www.ncbi.nlm.nih.gov/pubmed/36881761
http://dx.doi.org/10.1093/nar/gkad131

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