Cargando…
Effective extraction of polyribosomes exposes gene expression strategies in primary astrocytes
Regulation of mRNA translation in astrocytes gains a growing interest. However, until now, successful ribosome profiling of primary astrocytes has not been reported. Here, we optimized the standard ‘polysome profiling’ method and generated an effective protocol for polyribosome extraction, which ena...
| Autores principales: | , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2023
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123121/ https://www.ncbi.nlm.nih.gov/pubmed/36881761 http://dx.doi.org/10.1093/nar/gkad131 |