Protocol for in vitro fluorescence assay of papain-like protease and cell-based immunofluorescence assay of coronavirus infection

Here, we describe detailed steps to constitute an in vitro assay for monitoring papain-like protease of coronavirus and a cell-based immunofluorescence infection assay. These assays can be adapted for high-throughput screening to determine the efficacy of novel protease inhibitors of coronaviruses a...

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Detalles Bibliográficos
Autores principales: Chang, JuOae, Kim, Jinhee, Hong, Subin, Jeong, Kwiwan, Kim, Seungtaek, Lee, Wonsik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123355/
https://www.ncbi.nlm.nih.gov/pubmed/37167057
http://dx.doi.org/10.1016/j.xpro.2023.102295

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10123355/
https://www.ncbi.nlm.nih.gov/pubmed/37167057
http://dx.doi.org/10.1016/j.xpro.2023.102295

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