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Full confluency, serum starvation, and roscovitine for inducing arrest in the G(0)/G(1) phase of the cell cycle in puma skin-derived fibroblast lines
The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~10...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124155/ https://www.ncbi.nlm.nih.gov/pubmed/37101424 http://dx.doi.org/10.1590/1984-3143-AR2023-0017 |