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Full confluency, serum starvation, and roscovitine for inducing arrest in the G(0)/G(1) phase of the cell cycle in puma skin-derived fibroblast lines

The puma population is constantly decreasing, and cloning by somatic cell nuclear transfer can be used to conserve the species. One of the factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We evaluated the effects of full confluency (~10...

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Detalles Bibliográficos
Autores principales: Rodrigues, Luanna Lorenna Vieira, Moura, Yasmin Beatriz França, Viana, João Vitor da Silva, de Oliveira, Lhara Ricarliany Medeiros, Praxedes, Érika Almeida, Vieira, José de Brito, Sales, Sarah Leyenne Alves, Silva, Herlon Victor Rodrigues, Luciano, Maria Claudia dos Santos, Pessoa, Claudia, Pereira, Alexsandra Fernandes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Colégio Brasileiro de Reprodução Animal 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10124155/
https://www.ncbi.nlm.nih.gov/pubmed/37101424
http://dx.doi.org/10.1590/1984-3143-AR2023-0017