DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice

The global SARS-CoV-2 pandemic caused significant social and economic disruption worldwide, despite highly effective vaccines being developed at an unprecedented speed. Because the first licensed vaccines target only single B-cell antigens, antigenic drift could lead to loss of efficacy against emer...

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Autores principales: Persson, Gry, Restori, Katherine H., Emdrup, Julie Hincheli, Schussek, Sophie, Klausen, Michael Schantz, Nicol, McKayla J., Katkere, Bhuvana, Rønø, Birgitte, Kirimanjeswara, Girish, Sørensen, Anders Bundgaard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Immunology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126292/
https://www.ncbi.nlm.nih.gov/pubmed/37114047
http://dx.doi.org/10.3389/fimmu.2023.1166546
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author Persson, Gry
Restori, Katherine H.
Emdrup, Julie Hincheli
Schussek, Sophie
Klausen, Michael Schantz
Nicol, McKayla J.
Katkere, Bhuvana
Rønø, Birgitte
Kirimanjeswara, Girish
Sørensen, Anders Bundgaard
author_facet Persson, Gry
Restori, Katherine H.
Emdrup, Julie Hincheli
Schussek, Sophie
Klausen, Michael Schantz
Nicol, McKayla J.
Katkere, Bhuvana
Rønø, Birgitte
Kirimanjeswara, Girish
Sørensen, Anders Bundgaard
author_sort Persson, Gry
collection PubMed
description The global SARS-CoV-2 pandemic caused significant social and economic disruption worldwide, despite highly effective vaccines being developed at an unprecedented speed. Because the first licensed vaccines target only single B-cell antigens, antigenic drift could lead to loss of efficacy against emerging SARS-CoV-2 variants. Improving B-cell vaccines by including multiple T-cell epitopes could solve this problem. Here, we show that in silico predicted MHC class I/II ligands induce robust T-cell responses and protect against severe disease in genetically modified K18-hACE2/BL6 mice susceptible to SARS-CoV-2 infection.
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spelling pubmed-101262922023-04-26 DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice Persson, Gry Restori, Katherine H. Emdrup, Julie Hincheli Schussek, Sophie Klausen, Michael Schantz Nicol, McKayla J. Katkere, Bhuvana Rønø, Birgitte Kirimanjeswara, Girish Sørensen, Anders Bundgaard Front Immunol Immunology The global SARS-CoV-2 pandemic caused significant social and economic disruption worldwide, despite highly effective vaccines being developed at an unprecedented speed. Because the first licensed vaccines target only single B-cell antigens, antigenic drift could lead to loss of efficacy against emerging SARS-CoV-2 variants. Improving B-cell vaccines by including multiple T-cell epitopes could solve this problem. Here, we show that in silico predicted MHC class I/II ligands induce robust T-cell responses and protect against severe disease in genetically modified K18-hACE2/BL6 mice susceptible to SARS-CoV-2 infection. Frontiers Media S.A. 2023-04-11 /pmc/articles/PMC10126292/ /pubmed/37114047 http://dx.doi.org/10.3389/fimmu.2023.1166546 Text en Copyright © 2023 Persson, Restori, Emdrup, Schussek, Klausen, Nicol, Katkere, Rønø, Kirimanjeswara and Sørensen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Persson, Gry
Restori, Katherine H.
Emdrup, Julie Hincheli
Schussek, Sophie
Klausen, Michael Schantz
Nicol, McKayla J.
Katkere, Bhuvana
Rønø, Birgitte
Kirimanjeswara, Girish
Sørensen, Anders Bundgaard
DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title_full DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title_fullStr DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title_full_unstemmed DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title_short DNA immunization with in silico predicted T-cell epitopes protects against lethal SARS-CoV-2 infection in K18-hACE2 mice
title_sort dna immunization with in silico predicted t-cell epitopes protects against lethal sars-cov-2 infection in k18-hace2 mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10126292/
https://www.ncbi.nlm.nih.gov/pubmed/37114047
http://dx.doi.org/10.3389/fimmu.2023.1166546
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