CRISPR-Cas effector specificity and cleavage site determine phage escape outcomes

CRISPR-mediated interference relies on complementarity between a guiding CRISPR RNA (crRNA) and target nucleic acids to provide defense against bacteriophage. Phages escape CRISPR-based immunity mainly through mutations in the protospacer adjacent motif (PAM) and seed regions. However, previous spec...

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Detalles Bibliográficos
Autores principales: Schelling, Michael A., Nguyen, Giang T., Sashital, Dipali G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132644/
https://www.ncbi.nlm.nih.gov/pubmed/37058530
http://dx.doi.org/10.1371/journal.pbio.3002065

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10132644/
https://www.ncbi.nlm.nih.gov/pubmed/37058530
http://dx.doi.org/10.1371/journal.pbio.3002065

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