Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription

Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively. Herein, we developed a simple, low-background, and label-free...

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Detalles Bibliográficos
Autores principales: Si, Jingyi, Zhou, Wei, Fang, Ying, Zhou, Da, Gao, Yifan, Yao, Qunyan, Shen, Xizhong, Zhu, Changfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135927/
https://www.ncbi.nlm.nih.gov/pubmed/37185524
http://dx.doi.org/10.3390/bios13040449

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10135927/
https://www.ncbi.nlm.nih.gov/pubmed/37185524
http://dx.doi.org/10.3390/bios13040449

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