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Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells

Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of Rehmannia glutinosa polysaccharides (RGP) is optimized using two methods—the AB-8 macroporous resin (RGP-1) method and the H(2)O(2) (RGP-2) method. The optimal decolorization para...

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Autores principales: Ren, Heng, Li, Kejie, Min, Yan, Qiu, Binhang, Huang, Xiaolu, Luo, Jingxin, Qi, Liwen, Kang, Maoli, Xia, Peng, Qiao, Hanzhen, Chen, Jun, Cui, Yaoming, Gan, Liping, Wang, Peng, Wang, Jinrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136223/
https://www.ncbi.nlm.nih.gov/pubmed/37107289
http://dx.doi.org/10.3390/antiox12040914
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author Ren, Heng
Li, Kejie
Min, Yan
Qiu, Binhang
Huang, Xiaolu
Luo, Jingxin
Qi, Liwen
Kang, Maoli
Xia, Peng
Qiao, Hanzhen
Chen, Jun
Cui, Yaoming
Gan, Liping
Wang, Peng
Wang, Jinrong
author_facet Ren, Heng
Li, Kejie
Min, Yan
Qiu, Binhang
Huang, Xiaolu
Luo, Jingxin
Qi, Liwen
Kang, Maoli
Xia, Peng
Qiao, Hanzhen
Chen, Jun
Cui, Yaoming
Gan, Liping
Wang, Peng
Wang, Jinrong
author_sort Ren, Heng
collection PubMed
description Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of Rehmannia glutinosa polysaccharides (RGP) is optimized using two methods—the AB-8 macroporous resin (RGP-1) method and the H(2)O(2) (RGP-2) method. The optimal decolorization parameters for the AB-8 macroporous resin method were as follows: temperature, 50 °C; macroporous resin addition, 8.4%; decolorization duration, 64 min; and pH, 5. Under these conditions, the overall score was 65.29 ± 3.4%. The optimal decolorization conditions for the H(2)O(2) method were as follows: temperature, 51 °C; H(2)O(2) addition, 9.5%; decolorization duration, 2 h; and pH, 8.6. Under these conditions, the overall score was 79.29 ± 4.8%. Two pure polysaccharides (RGP-1-A and RGP-2-A) were isolated from RGP-1 and RGP-2. Subsequently, their antioxidant and anti-inflammatory effects and mechanisms were evaluated. RGP treatment activated the Nrf2/Keap1 pathway and significantly increased the activity of antioxidant enzymes (p < 0.05). It also inhibited the expression of pro-inflammatory factors and suppressed the TLR4/NF-κB pathway (p < 0.05). RGP-1-A had a significantly better protective effect than RGP-2-A, likely owing to the sulfate and uronic groups it contains. Together, the findings indicate that RGP can act as a natural agent for the prevention of oxidation and inflammation-related diseases.
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spelling pubmed-101362232023-04-28 Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells Ren, Heng Li, Kejie Min, Yan Qiu, Binhang Huang, Xiaolu Luo, Jingxin Qi, Liwen Kang, Maoli Xia, Peng Qiao, Hanzhen Chen, Jun Cui, Yaoming Gan, Liping Wang, Peng Wang, Jinrong Antioxidants (Basel) Article Polysaccharide decolorization has a major effect on polysaccharide function. In the present study, the decolorization of Rehmannia glutinosa polysaccharides (RGP) is optimized using two methods—the AB-8 macroporous resin (RGP-1) method and the H(2)O(2) (RGP-2) method. The optimal decolorization parameters for the AB-8 macroporous resin method were as follows: temperature, 50 °C; macroporous resin addition, 8.4%; decolorization duration, 64 min; and pH, 5. Under these conditions, the overall score was 65.29 ± 3.4%. The optimal decolorization conditions for the H(2)O(2) method were as follows: temperature, 51 °C; H(2)O(2) addition, 9.5%; decolorization duration, 2 h; and pH, 8.6. Under these conditions, the overall score was 79.29 ± 4.8%. Two pure polysaccharides (RGP-1-A and RGP-2-A) were isolated from RGP-1 and RGP-2. Subsequently, their antioxidant and anti-inflammatory effects and mechanisms were evaluated. RGP treatment activated the Nrf2/Keap1 pathway and significantly increased the activity of antioxidant enzymes (p < 0.05). It also inhibited the expression of pro-inflammatory factors and suppressed the TLR4/NF-κB pathway (p < 0.05). RGP-1-A had a significantly better protective effect than RGP-2-A, likely owing to the sulfate and uronic groups it contains. Together, the findings indicate that RGP can act as a natural agent for the prevention of oxidation and inflammation-related diseases. MDPI 2023-04-12 /pmc/articles/PMC10136223/ /pubmed/37107289 http://dx.doi.org/10.3390/antiox12040914 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ren, Heng
Li, Kejie
Min, Yan
Qiu, Binhang
Huang, Xiaolu
Luo, Jingxin
Qi, Liwen
Kang, Maoli
Xia, Peng
Qiao, Hanzhen
Chen, Jun
Cui, Yaoming
Gan, Liping
Wang, Peng
Wang, Jinrong
Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title_full Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title_fullStr Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title_full_unstemmed Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title_short Rehmannia glutinosa Polysaccharides: Optimization of the Decolorization Process and Antioxidant and Anti-Inflammatory Effects in LPS-Stimulated Porcine Intestinal Epithelial Cells
title_sort rehmannia glutinosa polysaccharides: optimization of the decolorization process and antioxidant and anti-inflammatory effects in lps-stimulated porcine intestinal epithelial cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10136223/
https://www.ncbi.nlm.nih.gov/pubmed/37107289
http://dx.doi.org/10.3390/antiox12040914
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