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CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation

Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing followin...

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Autores principales: Yang, Lan, Li, Hao, Han, Yao, Song, Yingjie, Wei, Mingchen, Fang, Mengya, Sun, Yansong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138046/
https://www.ncbi.nlm.nih.gov/pubmed/37107564
http://dx.doi.org/10.3390/genes14040806
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author Yang, Lan
Li, Hao
Han, Yao
Song, Yingjie
Wei, Mingchen
Fang, Mengya
Sun, Yansong
author_facet Yang, Lan
Li, Hao
Han, Yao
Song, Yingjie
Wei, Mingchen
Fang, Mengya
Sun, Yansong
author_sort Yang, Lan
collection PubMed
description Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein—γH2AX—was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system.
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spelling pubmed-101380462023-04-28 CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation Yang, Lan Li, Hao Han, Yao Song, Yingjie Wei, Mingchen Fang, Mengya Sun, Yansong Genes (Basel) Article Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein—γH2AX—was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system. MDPI 2023-03-27 /pmc/articles/PMC10138046/ /pubmed/37107564 http://dx.doi.org/10.3390/genes14040806 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Lan
Li, Hao
Han, Yao
Song, Yingjie
Wei, Mingchen
Fang, Mengya
Sun, Yansong
CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title_full CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title_fullStr CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title_full_unstemmed CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title_short CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
title_sort crispr/cas9 gene editing system can alter gene expression and induce dna damage accumulation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138046/
https://www.ncbi.nlm.nih.gov/pubmed/37107564
http://dx.doi.org/10.3390/genes14040806
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