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CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation
Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing followin...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138046/ https://www.ncbi.nlm.nih.gov/pubmed/37107564 http://dx.doi.org/10.3390/genes14040806 |
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author | Yang, Lan Li, Hao Han, Yao Song, Yingjie Wei, Mingchen Fang, Mengya Sun, Yansong |
author_facet | Yang, Lan Li, Hao Han, Yao Song, Yingjie Wei, Mingchen Fang, Mengya Sun, Yansong |
author_sort | Yang, Lan |
collection | PubMed |
description | Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein—γH2AX—was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system. |
format | Online Article Text |
id | pubmed-10138046 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-101380462023-04-28 CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation Yang, Lan Li, Hao Han, Yao Song, Yingjie Wei, Mingchen Fang, Mengya Sun, Yansong Genes (Basel) Article Clustered regularly interspaced short palindromic repeats (CRISPR) and the associated protein (Cas) gene editing can induce P53 activation, large genome fragment deletions, and chromosomal structural variations. Here, gene expression was detected in host cells using transcriptome sequencing following CRISPR/Cas9 gene editing. We found that the gene editing reshaped the gene expression, and the number of differentially expressed genes was correlated with the gene editing efficiency. Moreover, we found that alternative splicing occurred at random sites and that targeting a single site for gene editing may not result in the formation of fusion genes. Further, gene ontology and KEGG enrichment analysis showed that gene editing altered the fundamental biological processes and pathways associated with diseases. Finally, we found that cell growth was not affected; however, the DNA damage response protein—γH2AX—was activated. This study revealed that CRISPR/Cas9 gene editing may induce cancer-related changes and provided basic data for research on the safety risks associated with the use of the CRISPR/Cas9 system. MDPI 2023-03-27 /pmc/articles/PMC10138046/ /pubmed/37107564 http://dx.doi.org/10.3390/genes14040806 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Lan Li, Hao Han, Yao Song, Yingjie Wei, Mingchen Fang, Mengya Sun, Yansong CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title | CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title_full | CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title_fullStr | CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title_full_unstemmed | CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title_short | CRISPR/Cas9 Gene Editing System Can Alter Gene Expression and Induce DNA Damage Accumulation |
title_sort | crispr/cas9 gene editing system can alter gene expression and induce dna damage accumulation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138046/ https://www.ncbi.nlm.nih.gov/pubmed/37107564 http://dx.doi.org/10.3390/genes14040806 |
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