Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS

Isopropoxy benzene guanidine (IBG) is a guanidine derivative with antibacterial activity against multidrug-resistant bacteria. A few studies have revealed the metabolism of IBG in animals. The aim of the current study was to identify potential metabolic pathways and metabolites of IBG. The detection...

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Autores principales: Lu, Yixing, Zhang, Wanying, Zhang, Yongxiang, Wu, Sujuan, Ma, Minglang, Peng, Xianfeng, Zeng, Zhenling, Zeng, Dongping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138866/
https://www.ncbi.nlm.nih.gov/pubmed/37108473
http://dx.doi.org/10.3390/ijms24087313
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author Lu, Yixing
Zhang, Wanying
Zhang, Yongxiang
Wu, Sujuan
Ma, Minglang
Peng, Xianfeng
Zeng, Zhenling
Zeng, Dongping
author_facet Lu, Yixing
Zhang, Wanying
Zhang, Yongxiang
Wu, Sujuan
Ma, Minglang
Peng, Xianfeng
Zeng, Zhenling
Zeng, Dongping
author_sort Lu, Yixing
collection PubMed
description Isopropoxy benzene guanidine (IBG) is a guanidine derivative with antibacterial activity against multidrug-resistant bacteria. A few studies have revealed the metabolism of IBG in animals. The aim of the current study was to identify potential metabolic pathways and metabolites of IBG. The detection and characterization of metabolites were performed with high-performance liquid chromatography tandem mass spectrometry (UHPLC-Q-TOF-MS/MS). Seven metabolites were identified from the microsomal incubated samples by using the UHPLC-Q-TOF-MS/MS system. The metabolic pathways of IBG in the rat liver microsomes involved O-dealkylation, oxygenation, cyclization, and hydrolysis. Hydroxylation was the main metabolic pathway of IBG in the liver microsomes. This research investigated the in vitro metabolism of IBG to provide a basis for the further pharmacology and toxicology of this compound.
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spelling pubmed-101388662023-04-28 Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS Lu, Yixing Zhang, Wanying Zhang, Yongxiang Wu, Sujuan Ma, Minglang Peng, Xianfeng Zeng, Zhenling Zeng, Dongping Int J Mol Sci Article Isopropoxy benzene guanidine (IBG) is a guanidine derivative with antibacterial activity against multidrug-resistant bacteria. A few studies have revealed the metabolism of IBG in animals. The aim of the current study was to identify potential metabolic pathways and metabolites of IBG. The detection and characterization of metabolites were performed with high-performance liquid chromatography tandem mass spectrometry (UHPLC-Q-TOF-MS/MS). Seven metabolites were identified from the microsomal incubated samples by using the UHPLC-Q-TOF-MS/MS system. The metabolic pathways of IBG in the rat liver microsomes involved O-dealkylation, oxygenation, cyclization, and hydrolysis. Hydroxylation was the main metabolic pathway of IBG in the liver microsomes. This research investigated the in vitro metabolism of IBG to provide a basis for the further pharmacology and toxicology of this compound. MDPI 2023-04-15 /pmc/articles/PMC10138866/ /pubmed/37108473 http://dx.doi.org/10.3390/ijms24087313 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lu, Yixing
Zhang, Wanying
Zhang, Yongxiang
Wu, Sujuan
Ma, Minglang
Peng, Xianfeng
Zeng, Zhenling
Zeng, Dongping
Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title_full Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title_fullStr Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title_full_unstemmed Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title_short Metabolite Identification of Isopropoxy Benzene Guanidine in Rat Liver Microsomes by Using UHPLC-Q-TOF-MS/MS
title_sort metabolite identification of isopropoxy benzene guanidine in rat liver microsomes by using uhplc-q-tof-ms/ms
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10138866/
https://www.ncbi.nlm.nih.gov/pubmed/37108473
http://dx.doi.org/10.3390/ijms24087313
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