Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation

Tyrosyl DNA phosphodiesterase (TDP1) is a DNA repair enzyme that hydrolyzes the phosphotyrosyl linkage between 3′-DNA-protein crosslinks such as stalled topoisomerase 1 cleavage complexes (Top1cc). Here, we present a fluorescence-resonance-energy-transfer-(FRET) based assay to estimate modulation of...

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Detalles Bibliográficos
Autores principales: Bhattacharjee, Sangheeta, Richardson, Julia M., Das, Benu Brata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139991/
https://www.ncbi.nlm.nih.gov/pubmed/37058403
http://dx.doi.org/10.1016/j.xpro.2023.102218

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139991/
https://www.ncbi.nlm.nih.gov/pubmed/37058403
http://dx.doi.org/10.1016/j.xpro.2023.102218

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