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Fluorescence-resonance-energy-transfer-based assay to estimate modulation of TDP1 activity through arginine methylation

Tyrosyl DNA phosphodiesterase (TDP1) is a DNA repair enzyme that hydrolyzes the phosphotyrosyl linkage between 3′-DNA-protein crosslinks such as stalled topoisomerase 1 cleavage complexes (Top1cc). Here, we present a fluorescence-resonance-energy-transfer-(FRET) based assay to estimate modulation of...

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Detalles Bibliográficos
Autores principales:	Bhattacharjee, Sangheeta, Richardson, Julia M., Das, Benu Brata
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10139991/ https://www.ncbi.nlm.nih.gov/pubmed/37058403 http://dx.doi.org/10.1016/j.xpro.2023.102218

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